CRLB and Light sheet development

February 25, 2020

by Abhijit Marar

  Fisher information and the Cramer-Rao Lower Bound (CRLB):

 

CRLB for a Gaussian PSF without noise (1000 photons collected by sensor):

CRLB for a Gaussian PSF with noise (Signal = 1000 photons, background = 400 photons):

CRLB for a SIDH without noise (1000 photons collected by sensor):

Optical setup:

 

Relationship between various parameters of the hologram and the distance between the wavefront modulating element and the camera (z_h):

 

 

CRLB for a SIDH without noise (1000 photons collected by sensor):

CRLB for a SIDH with noise (signal:1000 photons, background: 3 photons):

Light Sheet Development and alignment:

The parts for the light-sheet have been assembled and are in the process of alignment. Optical aberration caused due to the air-gap between the prism and slide seems to be distorting the beam. To test this hypothesis we placed another prism on top of the slide and reduced the air-gap. This produced a perfect beam at the sample plane.

 

Holography tests:

We also tried incoherent holography with the new illumination path and it showed an improvement in the signal to noise ratio of the hologram. Below is a comparison of a hologram of a 40 nm bead using the two configurations. Hologram using older configuration (left). Hologram using newer illumination (right).