October 10, 2019
by Abhijit Marar
Our collaborator Daichi Kamiyama purchased new CF568 dye which has a higher probability of attaching the primary antibody. This has allowed for better imaging of the myopodia. In addition to this, we have been trying to perform two-color sequential imaging of the growth-cone (hrp- AF647) and Fas2 protein present in the growth cone and have had some promising results. Shown below are two images of the Myopodia stained with the new CF 568 dye.
The images below show two-color sequential imaging of the growth cone labeled with HRP-AF647 and Fas2-CF568
Finalized light-sheet design:
Below is the final design of the light-sheet add-on to the STORM setup. We are currently in the process of buying the parts to assemble it.